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Semen Detection Kits

We offer two semen detection kits: InSite® and CheckMate.

InSite Semen Detection Kit


The InSite Semen Detection Kit can detect traces of semen on a woman's undergarment discharged after sexual intercourse, and up to 36 hours later.

The InSite Kit comes with two types of test strips: (1) acid phosphatase (AP) test strips, and (2) prostate specific antigen (PSA) test strips. These two test work together to provide evidence of semen on a woman's undergarment.

The AP test strips can detect semen down to a 1/2000 dilution and provide instant results. The PSA test strips can detect semen to a 1/500,000 dilution and provide more sensitive results in just 10 minutes.

The InSite Semen Detection Kit contains 15 AP test strips in a resealable pouch with desiccant, 10 PSA test strips in sealed individual pouches and a 5-mL dropper.

How It Works

When a man has sexual intercourse with a woman, semen is deposited into the woman's vagina.  Immediately after intercourse, most of the semen flows back out, but some is retained in the vagina and slowly is discharged over a period of several days. 

Semen has over 900 identified proteins among which are semenogelin I and II (gel-forming proteins produced by the seminal vesicles), prostate-specific antigen (a protease which breaks down semenogelin), and acid phosphatase (which breaks down spermatozoa cell membranes). These proteins can be identified by immunochromatographic assay, which forms the principle of the PSA test in the InSite kit. 

Acid phosphatase can be detected by the classic test first described by Babson, which forms the principle of the AP test in the InSite kit.  This test relies on the catalytic hydrolysis of 1-naphthyl phosphate to form 1-naphthol, which in turn reacts with an aryl diazonium salt, forming an intensely colored azo dyestuff.  In addition to proteins, semen also has unusually high concentrations of zinc (100-200 mg/L v. 1 mg/L in plasma).  Zinc acts to stabilize DNA inside spermatozoa and also may catalyze the gel-forming reaction between semenogelin I and II.  Semen may be detected by the modified zinc test of Hooft and van de Voorde, but this test does not give as dramatic a color change at low dilutions as the AP test, and therefore the latter was chosen for inclusion in the InSite kit.

The semen flowing back out of a woman's vagina ("backflow") is deposited on her underwear or absorbent pad.  These items conveniently can be tested with the InSite kit.

Sensitivity and Accuracy

Semen may be detected on women's undergarments which has been discharged up to 17 hours after intercourse with the AP test strips, and up to 36 hours after intercourse with the PSA test strips.  The AP test is presumptive, and a positive result should always be followed by a PSA test for confirmation of the presence of semen.

The PSA test is 100% specific for semen at a dilution of 1/500,000 or less.  This means that the probability of a false positive result is essentially zero within the first 12 hours after intercourse.  In order to be sure that the semen did not come from you, you must not have had intercourse with your spouse for at least three days prior to the test.

PSA has been detected in semen stains on garments over 30 years old.

$69.95 (+ shipping and handling)

Total Price with Shipping Options

US Standard: 2 day USPS Priority*
US Overnight: FedEx Standard Overnight
International Standard: USPS Priority International (6-10 days*)
International Overnight: FedEx Overnight

Local Pick-up: If you are in the Southern California area, call us at toll-free 1-877-786-9323 to arrange a pick-up at our office.


CheckMate: The 5 Minute Infidelity Test


Checkmate is scientifically formulated to monitor your partner's sexual activity outside of the relationship by detecting traces of semen that are left in the undergarments, bed sheets, etc. after sex.

If they did it, it's there. Be the first to know instead of the last.

If you need to find out if someone is "cheating," this is the easiest, fastest, and most cost effective method ever invented.

Be prepared for the results in only 5 minutes.

Key Features of CheckMate

  • Quick and easy multiple use kit performs 10-12 tests

  • Results in 5 minutes or less

  • Designed to work on any type or color of material

  • Will not damage or stain the material being tested

  • CheckMate is guaranteed 100% effective

How It Works

CheckMate is scientifically formulated to detect a certain enzyme that is produced by the male prostate gland. This enzyme is found at very high levels in a man's semen. As long as the article to be tested has not been washed, invisible traces of dried semen will remain present for long periods of time--up to 2 years or even longer. If any semen is present, even the smallest trace amounts, you will see a purple color appear instantly.

For Men. A man will continue to secrete small amounts of semen for up to 2 hours after each sexual encounter unto his undergarments, bed sheets, etc.

For Women. A women will continue to secrete small amounts of semen for up to 72 hours after each sex act--even after showering. Women also experience "Flowback" in which dried and/or visible traces of dried semen deposit on to her undergarments.

See a Step-by-Step Demonstration


$49.95 (+ shipping and handling)


CheckMate and UV Stain Detector orders are processed through DTT CO. by our parent company DNA PLUS.

Order via phone at toll-free 1-877-786-9323 (24 hours)

Order via the web using a Credit Card (discreetly billed) Temporarily Backordered

Local Pick-up: If you are in the Southern California area, call us at toll-free 1-877-786-9323 to arrange a pick-up at our office.

If you do find semen, you can have it analyzed to see whose semen it is (or isn't).  Click here for more information.

UV Stain Detector

* appearance of stain detector may vary

Use the UV Stain Detector to isolate a stain before you test it.*

Order it with your InSite or CheckMate